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Flow Cytometry

Flow Cytometric Analysis is an indispensable tool used to clinically measure cell surface antigens, intracellular antigens, and DNA content of cells. The Flow Cytometer rapidly quantitates monoclonal antibodies bound to cellular markers on thousands of individual cells and provides objective information to complement traditional histopathologic and morphologic evaluation. Flow cytometry is commonly used to evaluate malignancies.

In addition measurement of nuclear DNA content in certain tumors provides clinicians with prognostic information which may be useful in selecting appropriate treatment.

All final reports are telephoned or faxed as soon as they become available. Abnormalities are verbally transmitted to the referring physician as appropriate. A full, interpretive written report is then mailed. Our professional staff is available at any time for further consultation.

Services Offered

  • Cell surface and intracellular markers
  • Natural Killer cell analysis
  • Immunodeficiency, leukemia and lymphoma
  • Therapy specific antigens (CD20, CD25, CD33, CD52)
  • Cell cycle analysis - DNA content
  • Stem cell enumeration - CD34 for stem cell transplant
  • If cytogenetic or molecular studies are also requested, the sample will be referred to the appropriate section for analysis.


Pediatric ALL bone marrow sample evaluated at time of diagnosis (above) and after treatment (below). A residual lymphoblastic population is quantitated in red.

Cell suspensions prepared from peripheral blood, bone marrow, bladder washings, effusions and tumor masses can be evaluated for the characterization of the types of cells present through the selected use of nearly one hundred monoclonal antibodies.

In the analysis of your patients' specimens, we utilize expert hematopathologists to determine the morphological significance, we then identify the presence of any abnormal cell populations and the lineage of the abnormal cells.

We determine the relative proportions of other major cell lineages present. Following lineage identification, a specific analysis of the differentiation state of the abnormal cells is performed with a highly specific panel of monoclonal reagents formulated to provide the most definitive clinical information.

We also test for the presence of TdT and cytoplasmic markers, whenever it is informative. A final report is prepared with interpretive comments for your assessment.

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